Fructanase Mixture (Ultrapure, recombinant, liquid)

10 ml / 20,000 Units

Item No.  E-FRLQPU

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High purity Fructanase Mixture (liquid) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

For Fructan Determination.

Fructanase, Amyloglucosidase (E-AMGDF) and Isoamylase (E-ISAMY) are used in the enzyme hydrolysis step of the AOAC method 2001.11 for the determination of polydextrose (a low molar mass dietary fiber) in foods.

Componentsexo-Inulinase (2,000 U/mL on kestose, at 40°C) and endo-Inulinase (100 U/mL on inulin at 40°C).

Note: This product is a mixture of two ultrapure, recombinant enzymes. Suitable for the measurement of fructan (AOAC Method 997.08) and in the measurement of Polydextrose (AOAC Method 2000.11).

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Specifications
Brand Megazyme
EC Number endo-Inulinase: 3.2.1.7,
exo-Inulinase: 3.2.1.80
Enzyme Fructanase
Storage Conditions

Storage Temperature: Below -10°C

Stability: Minimum 1 year at < -10°C. Check vial for details

Application Examples Applications in carbohydrate research and in the food industry.
Formulation In 50% (v/v) glycerol plus 0.02% sodium azide
Physical Form Solution
CAS Number 9001-57-4,
9025-67-6,
37288-56-5
CAZy Family GH32
Concentration endo-inulinase: Supplied at ~ 1,000 U
exo-inulinase: Supplied at ~ 10,000 U
Synonyms endo-inulinase: 1-beta-D-fructan fructanohydrolase
exo-inulinase: fructan β-fructosidase
Source Aspergillus niger
Expression Recombinant from Aspergillus niger
Specificity endo-Inulinase: endo-acting hydrolysis of (2,1)-β-D-fructosidic linkages in inulin.
exo-Inulinase: Hydrolysis of terminal, non-reducing β-D-fructofuranoside residues in β-D-fructofuranosides.
Unit Definition endo-Inulinase: One Unit of endo-inulinase activity is defined as the amount of enzyme required to release one μmole of β-D-fructose reducing-sugar equivalents per minute from inulin (20 mg/mL) in sodium acetate buffer (100 mM), pH 4.5.
exo-Inulinase: One Unit of exo-inulinase activity is defined as the amount of enzyme required to release one μmole of β-D-fructose reducing-sugar equivalents per minute from kestose (5 mg/mL) in sodium acetate buffer (100 mM), at pH 4.5 at 40°C.
Temperature Optima (°C) 60
pH Optima 4.5-4.9
Package Dimensions 0.80 in x 0.80 in x 1.90 in
Package Weight 0.06 lb

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