Fructanase Mixture (liquid)

10 mL / 20,000 Units

Item No.  E-FRMXLQ

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High purity Fructanase Mixture (liquid) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

For Fructan Determination (Liquid).

Fructanase, Amyloglucosidase (E-AMGDF) and Isoamylase (E-ISAMY) are used in the enzyme hydrolysis step of the AOAC method 2001.11 for the determination of polydextrose (a low molar mass dietary fiber) in foods.

Components: exo-inulinase 2,000 U/ml (on kestose, at 40°C), endo-inulinase ˜ 100 U/ml (on fructan at 40°C), α-galactosidase < 0.08 U/ml (on polygalacturonic acid at 40°C), β-Glucanase < 0.04 U/ml (on β-glucan at 40°C) and pectinase < 0.07 U/ml (on pectin at 40°C).

Note: This product has been purified to remove α-galactosidase, β-glucanase and pectinase which interfere with the use of the preparation in the measurement of fructan (AOAC Method 997.08) and in the measurement of Polydextrose (AOAC Method 2000.11).

Fructanase and sucrase (E-SUCR) are used in are used in the enzyme hydrolysis step of the AOAC method 2016.06 and the Chinese GB Standard 5009.255-2016 for the determination of fructan in foods.

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Specifications
Approvals AOAC Method 997.08, AOAC Method 2000.11, AOAC Method 2016.06, GB Standard 5009.245-2016 and GB Standard 5009.255-2016
Brand Megazyme
CAS Number 9001-57-4,
9025-67-6,
37288-56-5
CAZy Family GH32
EC Number endo-Inulinase: 3.2.1.7,
exo-Inulinase: 3.2.1.80
Enzyme Fructanase
Expression From Aspergillus sp.
Formulation In 50% (v/v) glycerol
Package Dimensions 0.80 IN x 0.80 IN x 1.90 IN
Package Weight 0.06 LB
pH Optima 4.5-4.9
Physical Form Solution
Source Aspergillus sp.
Specificity endo-Inulinase: endo-acting hydrolysis of (2,1)-β-D-fructosidic linkages in inulin.
exo-Inulinase: Hydrolysis of terminal, non-reducing β-D-fructofuranoside residues in β-D-fructofuranosides.
Storage Conditions

Storage Temperature: Below -10°C

Stability: Minimum 1 year at < -10°C. Check vial for details

Synonyms endo-inulinase: 1-beta-D-fructan fructanohydrolase
exo-inulinase: fructan β-fructosidase
Temperature Optima (°C) 40
Unit Definition endo-Inulinase: One Unit of endo-inulinase activity is defined as the amount of enzyme required to release one μmole of β-D-fructose reducing-sugar equivalents per minute from inulin (20 mg/mL) in sodium acetate buffer (100 mM), pH 4.5.
exo-Inulinase: One Unit of exo-inulinase activity is defined as the amount of enzyme required to release one μmole of β-D-fructose reducing-sugar equivalents per minute from kestose (5 mg/mL) in sodium acetate buffer (100 mM), at pH 4.5 at 40°C.

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