exo-Inulinase (Aspergillus niger)
5,000 Units at 40°C
Item No. E-EXOIAN
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High purity recombinant exo-Inulinase (Aspergillus niger) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
| Specifications | ||
|---|---|---|
| Brand | Megazyme | |
| EC Number |
3.2.1.80, 3.2.1.26 |
|
| Enzyme | β-Fructosidase | |
| Storage Conditions |
Storage Temperature: 2-8°C Stability: > 4 years at 4°C |
|
| Application Examples | Applications established in food industry for fructose syrup production and in the diagnostics industry for the measurement of fructans and inulins. | |
| Formulation | In 3.2 M ammonium sulphate | |
| Physical Form | Suspension | |
| CAS Number |
37288-56-5, 9001-57-4 |
|
| CAZy Family | GH32 | |
| Concentration | ~ 2,000 U/mL (40°C, pH 4.5 on kestose) | |
| Synonyms | fructan beta-fructosidase; beta-D-fructan fructohydrolase, beta-fructofuranosidase; beta-D-fructofuranoside fructohydrolase | |
| Source | Aspergillus niger | |
| Molecular Weight | 58400 | |
| Expression | Recombinant from Aspergillus niger | |
| Specificity |
EC 3.2.1.80: Hydrolysis of terminal, non-reducing (2,1)- and (2,6)-linked β-D-fructofuranose residues in fructans. EC 3.2.1.26: Hydrolysis of terminal, non-reducing β-D-fructofuranoside residues in β-D-fructofuranosides. |
|
| Specific Activity |
~ 2,000 U/mg (60°C, pH 4.5 on kestose); ~ 1,000 U/mg (40°C, pH 4.5 on kestose) |
|
| Unit Definition | One Unit of exo-inulinase activity is defined as the amount of enzyme required to release one μmole of β-D-fructose reducing-sugar equivalents per minute from kestose (5 mg/mL) in sodium acetate buffer (100 mM), at pH 4.5 at 40°C. | |
| Temperature Optima (°C) | 60 | |
| pH Optima | 4.5-4.9 | |
| Package Weight | 0.03 | |