exo-1,3-β-D-Glucanase + β-Glucosidase
300 Units exo-1,3-β-glucanase / 60 Units β-glucosidase
Item No. E-EXBGOS
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High purity exo-1,3-β-D-Glucanase (Trichoderma sp.) + β-Glucosidase (Aspergillus niger) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
| Specifications | ||
|---|---|---|
| Brand | Megazyme | |
| EC Number |
exo-1,3-β-glucanase: 3.2.1.58 β-glucosidase: 3.2.1.21 |
|
| Enzyme | exo-1,3-β-Glucanase, β-Glucosidase | |
| Storage Conditions |
Storage Temperature: 2-8°C Stability: Minimum 1 year at 4°C. Check vial for details. |
|
| Application Examples | For use in the determination of (1,3)(1,4) β-glucan. | |
| Formulation | In 3.2 M ammonium sulphate | |
| Physical Form | Suspension | |
| CAS Number |
exo-1,3-β-glucanase: 9073-49-8 β-glucosidase: 9001-22-3 |
|
| CAZy Family | GH55, GH3 | |
| Concentration | exo-1,3-β-Glucanase (100 U/mL) / β-Glucosidase (20 U/mL) | |
| Synonyms |
exo-1,3-β-Glucanase: glucan 1,3-beta-glucosidase; 3-beta-D-glucan glucohydrolase, β-Glucosidase: beta-glucosidase |
|
| Source | exo-1,3-β-Glucanase: Trichoderma virens, β-Glucosidase: Aspergillus niger | |
| Expression |
exo-1,3-β-D-Glucanase: Recombinant from Trichoderma sp. β-Glucosidase: Purified from Aspergillus niger |
|
| Specificity |
exo-1,3-β-glucanase: Successive hydrolysis of β-D-glucose units from the non-reducing ends of (1,3)-β-D-glucans, releasing α-glucose. β-glucosidase: Hydrolysis of terminal, non-reducing β-D-glucosyl residues with release of β-D-glucose. |
|
| Unit Definition |
exo-1,3-β-D-Glucanase: One Unit of exo-1,3-β-glucanase activity is defined as the amount of enzyme required to release one μmole of glucose reducing-sugar equivalents per minute from laminarin (10 mg/mL) in sodium acetate buffer (100 mM), pH 4.0 at 40°C. β-Glucosidase: One Unit of β-glucosidase activity is defined as the amount of enzyme required to release one μmole of p-nitrophenol per minute from 4-nitrophenyl-β-D-glucpyranoside in sodium acetate buffer (100 mM), pH 4.0 at 40°C. |
|
| Temperature Optima (°C) | 40 | |
| pH Optima | 4.0-4.4 | |
| Package Weight | 0.03 lb | |