Amyloglucosidase (Aspergillus niger) Powder
4 g - 36,000 Units/g
Item No. E-AMGDFPD
High purity Amyloglucosidase (A. niger) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
Amyloglucosidase. From A. niger. High purity. For use in Megazyme Total Starch and Dietary Fiber methods. Specifically prepared for use in AOAC Method 2001.03 which requires enzymes free of glycerol. This AMG is used in combination with thermostable α-amylase (Cat. No. E-BLAAM) and protease powder (Cat. No. E-BSPRPD). For use, the AMG is dissolved in water at a rate of 1 g/10 mL and 0.2 mL is used per assay. Electrophoretically homogeneous. Lyophilised powder.
Also suitable for use in AOAC 2001.03, AOAC Method 2000.11, AOAC Method 2016.06, GB Standards 5009.255-2016 and ISO Standard 15914:2004.
| Specifications | ||
|---|---|---|
| Brand | Megazyme | |
| Approvals | AOAC Method 2001.03, AOAC Method 2000.11, AOAC Method 2016.06, GB Standards 5009.255-2016 and ISO Standard 15914:2004 | |
| EC Number | 3.2.1.3 | |
| Enzyme | Amyloglucosidase | |
| Storage Conditions |
Storage Temperature: Below -10°C Stability: Minimum 1 year at < -10°C. Check vial for details |
|
| Formulation | Supplied as a lyophilised powder | |
| Physical Form | Powder | |
| CAZy Family | GH15 | |
| Synonyms | 4-alpha-D-glucan glucohydrolase; glucoamylase | |
| Source | Aspergillus niger | |
| Molecular Weight | 143500 | |
| Expression | From Aspergillus niger | |
| Specificity | Hydrolysis of terminal α-1,4 and α-1,6 D-glucose residues successively from non-reducing ends of maltodextrins. | |
| Specific Activity |
~ 36,000 U/g (40°C, pH 4.5, soluble starch as substrate); 3,100 U/g on p-nitrophenyl β-maltoside |
|
| Unit Definition | One Unit of amyloglucosidase activity is defined as the amount of enzyme required to release one μmole of D-glucose reducing-sugar equivalents per minute from soluble starch at pH 4.5 and 40°C. | |
| Temperature Optima (°C) | 70 | |
| pH Optima | 4.0-4.4 | |
| Package Weight | 0.04 lb | |
Documents
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