Amyloglucosidase (Aspergillus niger) Powder

4 g - 36,000 Units/g

Item No.  E-AMGDFPD

This item has shipping restrictions.
$255.00 each
In stock
Out of stock
Expect availability in 2-4 weeks
Qty:
Minimum quantity required: 1
This item must be ordered in multiples of one case. Quantity per case: 1

High purity Amyloglucosidase (A. niger) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

Amyloglucosidase. From A. niger. High purity. For use in Megazyme Total Starch and Dietary Fiber methods. Specifically prepared for use in AOAC Method 2001.03 which requires enzymes free of glycerol. This AMG is used in combination with thermostable α-amylase (Cat. No. E-BLAAM) and protease powder (Cat. No. E-BSPRPD). For use, the AMG is dissolved in water at a rate of 1 g/10 mL and 0.2 mL is used per assay. Electrophoretically homogeneous. Lyophilised powder.

Also suitable for use in AOAC 2001.03, AOAC Method 2000.11, AOAC Method 2016.06, GB Standards 5009.255-2016 and ISO Standard 15914:2004.

View Documents

Megazyme_Scheme-E-AMGDFPD.png
Specifications
Brand Megazyme
Approvals AOAC Method 2001.03, AOAC Method 2000.11, AOAC Method 2016.06, GB Standards 5009.255-2016 and ISO Standard 15914:2004
EC Number 3.2.1.3
Enzyme Amyloglucosidase
Storage Conditions

Storage Temperature: Below -10°C

Stability: Minimum 1 year at < -10°C. Check vial for details

Formulation Supplied as a lyophilised powder
Physical Form Powder
CAZy Family GH15
Synonyms 4-alpha-D-glucan glucohydrolase; glucoamylase
Source Aspergillus niger
Molecular Weight 143500
Expression From Aspergillus niger
Specificity Hydrolysis of terminal α-1,4 and α-1,6 D-glucose residues successively from non-reducing ends of maltodextrins.
Specific Activity ~ 36,000 U/g (40°C, pH 4.5, soluble starch as substrate);
3,100 U/g on p-nitrophenyl β-maltoside
Unit Definition One Unit of amyloglucosidase activity is defined as the amount of enzyme required to release one μmole of D-glucose reducing-sugar equivalents per minute from soluble starch at pH 4.5 and 40°C.
Temperature Optima (°C) 70
pH Optima 4.0-4.4
Package Dimensions 0.80 in x 0.80 in x 1.90 in
Package Weight 0.04 lb

Support