α-Amylase (Thermostable) (Bacillus sp.)

10 ml; 3,000 U/ml

SKU No.  700004204  |  Catalog No.  E-BSTAA

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High purity α-Amylase (Thermostable) (Bacillus sp.) for use in research, biochemical enzyme assays and in vitro diagnostic analysis. This enzyme is exceptionally thermostable and is the ideal choice for use in the total starch assay kits (K-TSTA and K-TSHK). It is considerably more thermostable than related enzymes such as the heat stable α-amylase (Bacillus licheniformis, E-BLAAM). Please see the data sheet for experimental stability data.

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Specifications
BrandMegazyme
EC Number3.2.1.1
Enzymeα-Amylase
Storage Conditions

Storage Temperature: Below -10°C

Stability: > 1 year under recommended storage conditions

Application ExamplesFor use in Megazyme Total Starch assay kits (K-TSTA and K-TSHK).
FormulationIn 50% (v/v) glycerol plus 0.02% sodium azide
Physical FormSolution
CAS Number9000-90-2,
9000-85-5
CAZy FamilyGH13
Concentration~ 3,000 U/mL
Synonymsalpha-amylase; 4-alpha-D-glucan glucanohydrolase
SourceBacillus sp.
Molecular Weight58000
ExpressionPurified from Bacillus sp.
SpecificityHydrolysis of α-1,4 glucosidic linkages in linear α-1,4 glucan (e.g. amylose regions in starch).
Specific Activity~ 170 U/mg (40°C, pH 6.5 on Ceralpha reagent).
Unit DefinitionOne Unit of α-amylase activity is defined as the amount of enzyme required to release one μmole of p-nitrophenol from blocked p-nitrophenyl-maltoheptaoside per minute (in the presence of excess α-glucosidase) at pH 6.5 and 40°C.
Temperature Optima (°C)100
pH Optima7
Package Dimensions0.80 in x 0.80 in x 1.90 in
Package Weight0.04 lb