Veratox® VIP for Walnut
SKU No. 700002601 | Catalog No. 8550
- Features an optimized antibody combination and extraction procedure for high specificity to all types of walnut protein, particularly further-processed walnut.
- Highly sensitive, quantifying walnut protein down to 0.15 ppm, aligned with most up-to-date industry guidelines.
- Rapid and easy to use, with a 30-minute time to result; a simple testing procedure consistent across sample types; and ready-to-use, non-hazardous reagents.
Qty:
Minimum quantity required: 1
This item must be ordered in multiples of one case. Quantity per case: 1
| Specifications | ||
|---|---|---|
| Brand | Veratox® | |
| Analyte | Walnut | |
| Platform | ELISA | |
| Result Type | Quantitative | |
| Limit of Detection | .03 ppm walnut protein | |
| Range of Quantitation | 0.15 ppm walnut - 3.75 ppm walnut | |
| Testing Time | 30.00 Minutes | |
| Storage Conditions | Store refrigerated | |
| Quantity per Package | 48 tests | |
| Package Weight | 1.00 lb | |
Additional Information
A Guide to Risk Assessment Using Food Allergen Reference DosesDocuments
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Technology
The Veratox VIP for Walnut test is a sandwich enzyme-linked immunosorbent assay (S-ELISA). This assay is validated to detect three primary allergenic proteins in walnuts. Walnut protein is extracted from samples with a buffer solution by shaking in a heated water bath, followed by centrifugation or filtration. Extracted walnut protein is sampled and added to antibody-coated wells (capture antibody), where it binds to the antibody during an incubation. Any unbound walnut protein is washed away and a second antibody (detector antibody), which is enzyme labeled, is added. The detector antibody binds to the already bound walnut protein. After a second wash, substrate is added. Color develops as a result of the presence of bound detector antibody. Red Stop Solution reagent is added and the color of the resulting solution is observed. The test is read in a microwell reader to yield optical densities (OD). The OD of the controls form a standard curve, and the sample OD are plotted against the curve to calculate the concentration of walnut protein.