1. 48 antibody-coated microwells
2. 48 red-marked transfer wells
3. 6 yellow-labeled bottles of 0, 2.5, 5, 10, 20, and 40 ppm gliadin controls (0, 5, 10, 20, 40, and 80 ng/mL gliadin)
4. 2 blue-labeled bottles of enzyme-labeled antibody conjugate
5. 1 green-labeled bottle of K-Blue^® Substrate
6. 1 red-labeled bottle of Red Stop solution
7. 40 mL of 10 mM PBS-Tween washing reagent in a widemouth bottle. Each bottle can prepare 1 L in distilled or deionized water (pH 7.4)
8. 1 foil pouch sample diluent concentrate of 10 mM PBS dry powder containing enough powder to prepare 1 L of dilution buffer
9. 50 g of extraction additive in 2 specimen cups
10. Plastic scoop to measure extraction additive

1. Microwell Reader with 650 nm filter (Neogen^® item 9303)
2. Graduated cylinder 10–20 mL (Neogen item 9447)
3. Graduated cylinder 250 mL (Neogen item 9368)
4. 1–Liter bottle (Neogen item 9472)
5. Pipettor, 50-200 µL adjustable (Neogen item 9276)
6. Pipettor, 12-channel 50-200 µL ((Neogen item 9273)
7. Pipette tips (Neogen item 9410, 9407, 9417)
8. Wash bottle (Neogen item 9400)
9. Well holder (Neogen item 9402)
10. Timer (Neogen item 9426)
11. Reagent boats, set of 3 (Neogen item 9435)
12. 7 mL tube or vial (Neogen item 9468)
13. Pipette pump for serological tubes (Neogen item 9277)
14. Pipettes, sterile serological, 10 mL (pk of 100) (Neogen item 9415)
15. Laboratory grade ethanol (190 proof)
16. Distilled water
17. Waterproof marker
18. Paper towel or equivalent
19. Centrifuge (optional)
20. Vortex (optional)

Non-heat treated method (extraction A or B)

1. Tube, 50 mL (Neogen item 9381) or bottle, 125 mL (Neogen item 9398)
2. Scale ± 0.1 g (Neogen item 9427, 9395)
3. Orbital rotator (Neogen item 9396) or shaker to hold 50 mL tube

Heat-treated cocktail method (extraction C)

1. Gliadin renaturing cocktail solution for heat processed samples (Neogen item 8515, 8515B, 8515S)
2. Orbital rotator (Neogen item 9396)
3. Scale, ± 0.01g (Neogen Item 9395)
4. Water bath or oven at 50°C