Reveal® for Total Milk Allergen is intended for the qualitative analysis of milk residue (casein or whey) in liquid products (e.g., juices and sorbet), clean-in-place rinses, and on environmental surfaces.
Analyte Milk
Brand Reveal®
Limit of Detection Screens samples at 5 ppm total milk ppm
Package Dimensions 8.40 IN x 5.10 IN x 2.50 IN
Package Weight 0.20 LB
Platform Lateral Flow
Quantity per Package 24 tests
Result Type Qualitative
Testing Time 5.00 Minutes

  1. 24 lateral flow milk test strips in a sealed stay-dry tube
  2. 24 reagent wells in a foil pouch
  3. 3 foil pouches of 10 mM PBS dry powder extraction solvent; each pouch contains enough powder to prepare 1 L of extraction solution
  4. 1 bottle of sample diluent
  5. 1 bottle of extraction additive
  6. Plastic 1 g scoop to measure extraction additive
  1. Water bath, hot plate or equivalent heat source capable of maintaining 60°C ± 1° (140°F)
  2. Thermometer
  3. 1 L bottle to prepare extraction solution (Neogen® item #9472)
  4. Pipettor, 100 µL (Neogen item #9272, 9278 or 9276)
  5. Pipette tips (Neogen item #9410, 9407)
  6. Well holder (Neogen item #9402)
  7. Waterproof marker
  8. Distilled or deionized water
  9. Timer (Neogen item #9426)
  10. Graduated cylinder capable of measuring 5-100 mL (Neogen item #9368)
  11. Allergen Extraction Kit (Neogen item #8429)
  12. Allergen Environmental Swabbing Kit (Neogen item #8432S)


Assay Principle

Reveal for Total Milk Allergen is a two part lateral flow immunochromatographic assay. The first part of the assay consists of a detector reagent well, which contains antibodies specific for milk residue conjugated to colored particles. This reagent is rehydrated with a supplied sample diluent, and sample is added to the reagent well. If milk residue is present in the sample, it will be bound by the antibody–particle complex.

The second part of the assay consists of an immunochromatographic device used for the detection of milk residue. This device is inserted into the sample/reagent well. The milk-antibody-particle complex is then wicked onto a membrane which contains a zone of antibody specific for milk residue. This zone captures the complex allowing the particles to concentrate and form a visible line. If no milk is present, no line will form. The membrane also contains a control zone where an immune complex present in the reagent zone is captured by an antibody, forming a visible line. The control line will always form regardless of the presence of milk, ensuring the strip is working properly.


Our customers’ success is our shared success. Our experts are ready to train you and your team on our solutions, so you can rest easy knowing procedures are performed properly and yield accurate results. In addition, we provide certificates upon completion of training to provide you with the documentation needed for audit traceability.



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