Hazelnut Protein ELISA Kit

SKU No.  700002318  |  Catalog No.  E96HZL

  • Test can accommodate environmental swab, CIP rinse water and food samples.
  • A universal extraction protocol streamlines testing for multiple analytes, saving you reagents and valuable time.
  • 3M™ Environmental Collection Swabs sold separately.
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$572.00 each
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Minimum quantity required: 1
This item must be ordered in multiples of one case. Quantity per case: 1
The Hazelnut Protein ELISA Kit is a microwell sandwich ELISA, used for the quantitative analysis of low levels of hazelnut residues in food products, ingredients, clean in place (CIP) rinses and environmental swabs in a 96 well format. The quantity of protein in the sample can be extrapolated from the standard curve, constructed from standards of know concentration, and adjusted to consider the sample dilution.
Specifications
Analyte Hazelnut
Platform ELISA
Result Type Quantitative
Range of Quantitation 1.00 ppm hazelnut protein - 81.00 ppm hazelnut protein
Testing Time 50.00 Minutes
Storage Conditions 36-46°F, 2-8°C
Quantity per Package 96 tests
Package Weight 1.13 lb
  1. 1 pouch containing 96 antibody-coated microwells
  2. 1 vial 10x hazelnut HRP conjugate
  3. 1 vial hazelnut protein standard concentrate
  4. 1 bottle 5x diluent
  5. 1 bottle 20x wash solution
  6. 1 bottle 4x extraction buffer E26
  7. 1 bottle substrate solution
  8. 1 bottle stop solution
  1. Shaker water bath or shaking incubator
  2. Precision pipettor and tips to collect 10 to 1000 uL
  3. Precision 8-channel pipettor
  4. Reagent reserviors
  5. Centrifuge tubes for protein extraction
  6. Microcentrifuge tubes for reagent preparation
  7. Centrifuge tube rack
  8. Environmental collection swabs
  9. Lab scale
  10. Weigh dishes
  11. Metal spatula
  12. Plate sealing film
  13. Centrifuge
  14. Laboratory grinder or blender
  15. Graduated cylinders
  16. Reagent bottles
  17. Wash bottles
  18. Timer
  19. Vortex
  20. Orbital shaker

Technology

The Hazelnut Protein ELISA kit utilize a sandwich ELISA. The target protein present in the sample react with the specific antibodies, which have been adsorbed to the surface of polystyrene microtiter wells. After the removal of unbound proteins by washing, antibodies conjugated with horseradish peroxidase (HRP) are added. This enzyme-labeled antibody forms complexes with the previously bound target protein. Following a second washing step, the enzyme bound to the immunosorbent is detected by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The color development from this enzymatic reaction varies directly with the concentration of target protein in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of the target protein in the test sample. The quantity of protein in the test sample can be extrapolated from the standard curve, constructed from standards of known concentration, and adjusted to consider the sample dilution.

Training

Our customers’ success is our shared success. Our experts are ready to train you and your team on our solutions, so you can rest easy knowing procedures are performed properly and yield accurate results. In addition, we provide certificates upon completion of training to provide you with the documentation needed for audit traceability.

Support

Validation

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