Alert® for Total Milk
SKU No.  700002579  | Catalog No.  8471
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Alert® for Total Milk

SKU No.  700002579  |  Catalog No.  8471

  • Minimal equipment required
  • Screen single or multiple samples at one time
  • Test food, rinses or swabs
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Qty:
Minimum quantity required: 1
This item must be ordered in multiples of one case. Quantity per case: 1
To order or for more information contact us
To order or for more information contact us
Alert® for Total Milk Allergen is used as a screening tool to test raw ingredients, in clean-in-place rinses, on environmental surfaces, or product throughout the production process, for the presence of milk residue.
Specifications
BrandAlert®
AnalyteMilk
PlatformELISA
Result TypeQualitative
Limit of Detection5 ppm, 10 ppm NFDM
Testing Time30.00
Prop 65 Warning
Warning
WARNING: This product can expose you to chemicals including thimerosal, which is known in the State of California to cause birth defects or other reproductive harm. For more information, go to www.P65Warnings.ca.gov.
Quantity per Package20 tests
Package Dimensions10.50 in x 8.00 in x 3.00 in
Package Weight0.60 lb

  1. 24 antibody-coated microwells
  2. 2 yellow-labeled bottles of controls made from nonfat dried milk; 1 bottle at a concentration of 5 ppm and 1 bottle at 10 ppm (see procedural note No. 1)
  3. 1 blue-labeled bottle of enzyme-labeled antibody conjugate
  4. 1 green-labeled dropper bottle of K-Blue® Substrate solution
  5. 1 red-labeled dropper bottle of Red Stop solution
  6. 20 unlabeled empty sample dropper bottles with tips
  7. 3 foil pouches of 10 mM PBS dry powder extraction solvent; each pouch contains enough powder to prepare 1 L of extraction solution
  8. 1 wide-mouth bottle of 40 mL PBS-Tween wash buffer concentrate; a bottle contains enough concentrate to prepare 1 L of wash buffer
  9. 50 g of extraction additive in a specimen cup
  10. Plastic scoop to measure extraction additive

  1. Allergen Extraction Kit (Neogen® item 8429)
    1. 20 disposable extraction bottles
    2. 20 disposable transfer pipettes
  2. Allergen Environmental Swabbing Kit (Neogen item 8432S)
    1. 100 sterile swabs
    2. 100 disposable dropper tips
  3. Scale capable of weighing 5 ± 0.1 g (Neogen item 9427)
  4. Shaker water bath, hot plate or equivalent heat source capable of maintaining 60 ± 1°C
  5. Thermometer
  6. Two 1 L bottles to prepare washing and extraction solutions (Neogen item 9472)
  7. Paper towels or equivalent absorbent material
  8. Microwell holder (Neogen item 9402)
  9. Waterproof marker
  10. Wash bottle (Neogen item 9400)
  11. Distilled or deionized water
  12. Timer (Neogen item 9426)
  13. Graduated cylinder capable of measuring 125 mL (Neogen item 9368)
  14. Pipettor, 100 µL (Neogen item 9272, 9278, 9276)
  15. Pipette tips (Neogen item 9410, 9407, 9417)
  16. Vortex (Neogen item 9494)
  17. 50 mL sample tubes (Neogen item 9381)

Documents

To access certificates, please use our technical document search page.

Alert® for Total Milk - Kit Insert - English - US

Alert® for Total Milk - Procedures - English - US

Alert® for Total Milk - English - US Alert® for Total Milk - Greek - Greece Alert® for Total Milk - Maltese - Malta Alert® for Total Milk - Spanish - US Alert® for Total Milk - Latvian - Latvia Alert® for Total Milk - Croatian - Croatia Alert® for Total Milk - English - Canada Alert® for Total Milk - French - Belgium Alert® for Total Milk - Norwegian - Norway Alert® for Total Milk - Japanese - Japan Alert® for Total Milk - Swedish - Sweden Alert® for Total Milk - French - Canada Alert® for Total Milk - Vietnamese - Vietnam Alert® for Total Milk - Finnish - Finland Alert® for Total Milk - French Alert® for Total Milk - Italian - Italy Alert® for Total Milk - Indonesian - Indonesia Alert® for Total Milk - Thai - Thailand Alert® for Total Milk - Danish - Denmark Alert® for Total Milk - Polish - Poland Alert® for Total Milk - English - New Zealand Alert® for Total Milk - German Alert® for Total Milk - German - Austria Alert® for Total Milk - English - Philippines Alert® for Total Milk - Estonian - Estonia Alert® for Total Milk - Dutch - Netherlands Alert® for Total Milk - Malay - Malaysia Alert® for Total Milk - Chinese Simplified - China Alert® for Total Milk - Hungarian - Hungary Alert® for Total Milk - Korean - Korea Alert® for Total Milk - English - EU/UK Alert® for Total Milk - Slovak - Slovakia Alert® for Total Milk - German - Luxembourg Alert® for Total Milk - Spanish - Mexico Alert® for Total Milk - Greek - Cyprus Alert® for Total Milk - English - Australia Alert® for Total Milk - Dutch - Belgium Alert® for Total Milk - Spanish - Colombia Alert® for Total Milk - Portuguese - Brazil Alert® for Total Milk - English - Ireland Alert® for Total Milk - Spanish - Spain Alert® for Total Milk - Lithuanian - Lithuania Alert® for Total Milk - English - Malta Alert® for Total Milk - Czech - Czech Republic Alert® for Total Milk - Chinese Traditional - Taiwan Alert® for Total Milk - Slovene - Slovenia Alert® for Total Milk - Bulgarian - Bulgaria Alert® for Total Milk - Portuguese - Portugal

Technology

First, samples and controls are added to their respective wells. During an incubation, the target substance binds to the antibodies. The wells are washed to remove all unbound materials.

An enzyme conjugate (antibody chemically linked with an enzyme) is added to all wells. During an incubation, conjugate binds to the already bound target substance, forming a “sandwich.” The more target substance in the wells, the more conjugate that binds in the wells.

The wells are washed again to remove all unbound conjugate.

A substrate, which changes color in the presence of the conjugate, is added to the wells. During an incubation, color develops in proportion to the amount of conjugate in the wells. The more conjugate, the more color, and the more target substance that is detected.

Results are read visually in a screening format—the more blue color, the more target substance detected.

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Training

Our customers’ success is our shared success. Our experts are ready to train you and your team on our solutions, so you can rest easy knowing procedures are performed properly and yield accurate results. In addition, we provide certificates upon completion of training to provide you with the documentation needed for audit traceability.

Support

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Validation

Our experts are here to help. If you need information beyond what is provided in Validation Reports under the Documents tab, please contact us and we will be happy to assist you. Learn more